Objectives
Dermal substitutes provide scaffold for autologous cells to reconstruct dermis. The micromolecular environment is different from granulation tissue and may affect the signaling cues during wound healing and final outcome. We compared the effect of three different wound bed compositions on STSGs protein content one year after operation. 
Methods
Test area of 10x15 cm of a large deep burn after excision of four patients was divided into three 10x5 cm test sections: one covered with STSG as a control group, one with artificial dermal substitute Integra and one with cellulose sponge as a temporary cover inducing granulation tissue. After two weeks the test sections treated with dermal substitute and cellulose sponge were also transplanted with STSGs. After one year biopsy samples were taken from each test section and analyzed with site-targeted proteomics and validated using immunohistochemistry to detect differences between treatments.
Results
Out of the 481 proteins identified 12 % were significantly differentially expressed (p< 0.05) between the different test sections; 30 proteins of epidermis and 24 proteins of dermis. The biggest difference between epidermal proteins was found in axonemal heavy chain dynein DNAH10 with 34-fold difference between the control group and the induced granulation tissue after cellulose sponge treatment. In dermis the four biggest differences were detected in expression of keratins KRT1, KRT6C, KRT16 and KRT77 likely from skin appendages; their expression was higher in induced granulation test section compared to other test sections.
Conclusion
Different dermal matrix compositions during early wound healing seem to have long term or permanent effects on split thickness skin grafts. The significance of DNAH10 for wound healing and epidermal maintenance requires further investigations.

8 - Split thickness skin grafts (STSGs) reflect differences in early composition of extracellular matrix on wound bed year later in proteomic analysis - a comparative study