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Mar 24, 2020

ACS Spring 2020 National Meeting & Expo

Acoustically active multifunctional nanomaterials improving stem cells therapy efficacy in myocardial infarcted mice

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Abstract

Stem cells have great potential in regenerative medicine but have had relatively low clinical efficacy due to mis-injections, low cell retention, and poor cell viability after transplantation. Here, we prepared a nanoparticle (SIO) to overcome these limitations: SIO served as a slow-release reservoir for pro-survival agent IGF-I. It also produced ultrasound and MRI signal for cell monitoring and helped immobilize cells in the affected area via a magnet. The viability of human mesenchymal stem cells (hMSCs) incubated with 1 mg/ml SIO was 99%, and a proliferation assay showed that the doubling time for labeled and unlabeled hMSCs were 2.31 and 2.27 days. The loading capacity was around 1.89 µg IGF/mg SIO. While IGF-loaded SIO (IGF@SIO) has a burst release in the first 12 hours, there was sustained release for up to one week (Fig. 1A). IGF@SIO increased the hMSCs survival for one week compared to free IGF (Fig. 1B). SIO-labeling increased ultrasound and MRI signals of hMSCs(Fig. 1C-E). Moreover, a magnet could direct SIO-labeled cell growth (Fig. 1F) and increased SIO retention in mice heart by approximately 77%. In vivo studies with a mouse model of myocardial infarction showed that IGF@SIO-labeled hMSCs significantly increased the heart functions compared to hMSCs only (Fig. 1G).

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© Copyright 2019 Morressier GmbH.
All rights reserved.