Effect of anaesthetic techniques on migration and apoptosis MDA-MB-231 tumor cells in vitro,
in breast cancer surgery
Author(s): Morís L., Cassinello F., Lopez V.
Institute(s): Fundacion Jimenez Diaz University Hospital, Dept of Anaesthesiology, Madrid, Spain
Text: Background: Some drugs and anaesthetic techniques are proving to have influence on the
proliferation, invasion and apoptosis of tumor cells.
Goal of Study: To compare the effect of serum obtained from patients with breast cancer who
received two different types of anaesthesia on migration and apoptosis tumor cells in vitro.
Materials and Methods: Patients undergoing mastectomy were randomized to receive
propofol/lateral and anterior intercostal nerve block anaesthesia(PBI,n=4)or sevoflurane/opioid
anaesthesia (SGA,n=4).
Blood samples:prior to induction,1h and 24h after the end of the surgery.
The ER-negative MDA-MB-231cell line was treated with patient serum.
The migration was detected through a wound healing scratch assay test.Apoptosis was evaluated
with fluorescence stained with annexin V and 7-AAD.
Results and Discussion: There was lower migration in the PBI group without differences
statistically significant.
[Migra1]
[Migra24]
. A reduction of apoptosis(39.8%)was observed in the SGA group(p 0.06)compared to the PBI
group(0.75%).The results are not significant.
[Apop]
Conclusions: Tumor cell line cultured with the serum of PBI patients migrate more slowly.Reduction
of apoptosis was observed in cell line cultured with serum of SGA group.
References:
Deegan CA et al.Effect of anaesthetic technique on oestrogen receptor-negative breast cancer cell
function in vitro. Br J Anaesth.2009;103(5):685-90.
Jaura AI et al.Differential effects of serum from patients administered distinct anaesthetic techniques
on apoptosis in breast cancer cells in vitro: a pilot study. Br J Anaesth.2014;113:i63-7

Effect of anaesthetic techniques on migration and apoptosis MDA-MB-231 tumor cells in vitro, in breast cancer surgery