Objectives: Authors’ very first experience with application of glycerol preserved allografts (GPAs) in acute burn treatment applied in a 7-year-old boy with 93% TBSA 3rd degree flame burn.

Methods: After excision of the necrotic tissue during the initial 4 days, INTEGRA DRT was applied on the extremities. Given the fact that the take rate of INTEGRA DRT was around 30%, allogenic skin grafts were imported from the Banc de Sang i Teixits - Barcelona and grafted onto the wound bed, 14 days after the patient’s admission. Donor sites on the scalp and the foot were covered with amniotic membranes (AM) from the local Tissue Bank. During 4 months of hospitalization, GPAs were applied 6 times (7,000cm2),

Results: AM promoted faster healing in small areas of IInd degree burns. Cadaveric skin grafts enabled the preservation of the wound bed for subsequent autologous skin grafting combined with cultured epithelial autografts (CEA). CEA were applied in preconfluent phase with two-component fibrin glue (Tisseel, Baxter).The take rate after wound bed conditioning with allogenic skin was around 90%. An episode of mold infection occurred on the area covered with allogenic skin what prompted immediate removal of the grafts and initiation of antifungal therapy.
Discussion:

Allogenic skin grafts provided a beneficial temporary wound dressing and enabled a very satisfying take rate of autologous skin grafts. Treatment of the patient is continuing with uncertain outcome.

Initial experience with the first application of allogenic skin grafts for acute burns in Croatia