Lysosomes are membrane-bound intracellular vesicles with a low pH and high concentration of enzymes. These organelles are essential for the processing of intracellular cargos ranging from nutrients to viruses. Our recent work with TiO2 nanoparticles shows, using a combination of fluorescence and electron microscopy, that these nanoparticles accumulate in lysosomes resulting in a 2-fold increase in lysosome diameter. We have used single particle tracking fluorescence microscopy to characterize the motion of these enlarged vesicles with the goal of understanding how increased lysosome diameter affects the global cellular response to TiO2 nanoparticles. Beyond nanoparticle-cell interactions, an understanding of lysosomal transport is important for fundamental cellular biophysics as well as human health and disease, specifically lysosomal storage disorders.

Intracellular transport of TiO2 nanoparticle-containing lysosomes

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.