The use of embryonated egg as an alternative in the study of the pathogenesis of fungi is evolving, although murine models are “gold standard” however, these models are used to screen determinants of virulence among fungi species. This study was aimed at the study of the virulence potential of Cryptococcus gattii strains R265, R272, EJB18 and Malassezia sympodialis via Chorio-allantioc membrane (CAM) in embryonated egg. At a concentration of 107 cfu/ml, C .gattii R272 was more virulent than R265 in the egg model while EJB18 had a low virulence. The CAM model supported the growth of Malassezia sympodialis strain and induced the formation of hyphae. The formation of lesions by the organism and re-isolation of organism from CAM suggests that the model can be used for evaluating the virulence of C.gattii. Histopathology of CAM from both strains revealed massive disruption of CAM Also . This study suggests that embryonated eggs is  a useful alternative tool to pre-screen Cryptococcus gattii  strains to select strains for subsequent testing in murine models and could be a potential medium for studying the hyphal growth in Malassezia species

Infection of chick chorioallantoic membrane (CAM) As a model for the pathogenesis of Cryptococcus Gatti