In this work we demonstrate the effectiveness of an engineered two-dimensional micropatterned cell culture platform for creating highly aligned myotubes from human induced pluripotent stem cell (iPSC)-derived myogenic progenitors. Myotube elongation was shown to be dependent on the micropattern feature width and spontaneous contractions were aligned with the long axis of the pattern.As a result of an optimized micropattern feature geometry on a substrate of physiologically relevant stiffness, the resulting myotubes were elongated, well-aligned, and similar to myofibers; they showed significant improvement innuclear alignment, myotube fusion, and sarcomere formation. This platform holds great potential in further understanding the process of human muscle development, as well as opportunities for <i>in vitro</i>pharmacological studies with skeletal muscle diseases. As an example, we demonstrated that bundle-like myotubes can be produced using patient-derived iPSCs with a background of Pompe disease (glycogen storage disease type II). Culturing in this engineered platform enhanced the disease phenotype as demonstrated by the observation of abnormal lysosome accumulations. <br/>

Maturation of stem cell-derived skeletal myocytes promoted by micropatterning and substrate stiffness

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.