When nanoparticles (NPs) encounter a biological fluid, proteins, lipids, and other biomolecules adsorb on the nanoscale surface consequently leading to the evolution of a protein shell or “corona”. The corona formed is dynamic in nature and depends on the “synthetic identity” of the NPs, thereby ultimately affecting their biological response. In this paper, an integrated microfluidic platform coupled with electrical resistance measurement setup is developed to monitor and investigate the real-time formation of a biomolecular corona of carbon nanoparticles and effectively discriminated “soft” vs. “hard” corona formation stages based on their nanoscale surface chemistries when combined with a time-frequency tool known as wavelet transform (WT) and machine-learning (ML) techniques. Additionally, the corona and its composition were studied using different techniques such as dynamic light scattering, nanoparticle tracking analysis, zeta potential, excitation-emission profiles, 1D SDS-PAGE and subsequently, LC-MS analysis. We further demonstrated the protein corona formation process influences the cellular internalization process and is also dependent on pH of the system, paving a way for synthesizing robust pH-controlled drug delivery system. Our dynamic setup has paved a potential avenue in controlling the adsorption of proteins on NPs by tailoring the chemical motifs at its surface could be a valuable tool with promising therapeutic benefits in drug delivery and targeted cancer treatment in the near future<br/><br/><br/><br/>

Machine-learning and wavelet transform assisted on-chip electrical monitoring of real-time “soft” and “hard” protein corona formation in carbon nanoparticles

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.