Self-assembled DNA nanostructures have been demonstrated to organize multienzyme systems with controlled geometric patterns. For example, DNA nanostructures were used to mediate the proximity assembly of enzyme cascades with enhanced and regulated activities. In this study, we assembled an enzyme pair of pyruvate kinase (PK) and hexokinase (HEK) with controlled distances on a rationally designed DNA origami. To facilitate the transport of ATP cofactors between the two enzymes, we modified the surface of a DNA origami with ATP-binding affinity to attract ATP molecules, and guide their diffusion to target enzymes. Significant activity enhancement was observed with the introduction of ATP-binding affinity channel between enzymes, as compared with enzymes without this channel. To fully understand the relationship between ATP aptamer and PK/HEK, a theoretical model also built to explain this ATP aptamer behavior.

Self-assembly of an enzyme cascade with ATP channeling on DNA nanostructures

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.