Tuberculosis is one of the world’s leading cause of mortality from a single bacterial pathogen, with over 10 million reported cases each year. There is an alarming increase in the prevalence of drug-resistant strains, thus the need for the discovery of novel anti-tubercular agents. Mycobacterium tuberculosis shikimate kinase (MtSK) catalyzes the 5th step of the shikimate pathway, converting shikimate to shikimate-3-phosphate. The overall goal of this project is to express and characterize MtSK and screen for potential anti-tubercular agents. Transformation of XL-1 blue competent cells was performed using a pET 21b plasmid with aroK gene inserted at the multiple cloning site. Plasmids were cloned and purified and used to transform BL 21 DE3 competent cells for subsequent protein expression. With further small and large scale expression, purification and characterization, MtSK kinetic parameters would be determined prior to enzyme inhibition studies using inhibitors like avarone, a marine sponge sesquiterpene quinone and derivatives thereof.

Shikimate kinase: Potential target for a new antibacterial agents

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.