With the recent increase in early phase cell therapy clinical trials, there is a need for a manufacturing platform which can be implemented in research labs and easily scaled up to expedite process development studies, pre-clinical testing, and large-scale expansion of adherent human cells. Although various platforms such as traditional flatware and stirred tank bioreactors with microcarriers exist and have been well characterized, traditional flatware often does not allow for tight control of the cellular environment and is not scalable. Pall’s Xpansion single-use bioreactor offers a tightly-controlled, scalable manufacturing platform for cell therapy applications, allowing for expedited process development, pre-clinical testing and large scale, high-density expansion of progenitor cells while maintaining their differentiation capacity. We have previously demonstrated efficient expansion of both epithelial cells (Vero, HEK293) and human mesenchymal stem/stromal (hMSC) in the Xpansion single-use bioreactor. Here we extend these findings by successfully expanding a proprietary progenitor cell to extremely high cell densities (&gt; 1 million cells/cm2). The first stage of this process begins with a pluripotent stem cell line, which is expanded and differentiated into proprietary lineage-specific progenitor cells. We then demonstrate growth and differentiation of this progenitor cell in the Xpansion bioreactor using a 14-day and 21-day protocol. In summary, both protocols resulted in 1) cell expansion to &gt; 1 million cells/cm2 and equivalent cell densities to the traditional flatware process control; 2) equivalent differentiation to a more mature cell fate as indicated by expression of key cell surface markers; and 3) self-assembly into characteristic three-dimensional structures.

Pall Xpansion® bioreactor supports progenitor cell growth to >1 million cells/cm2 and proper cell differentiation

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.