Plant natural products are important sources of pharmaceuticals, with more than 10% of the WHO listed essential medicines of flowering plant origin. However, the biosynthetic pathways of most phytochemicals are not fully elucidated - enzymes catalyzing certain steps in the proposed pathway are unknown. Without the comprehensive understanding of the biosynthesis of plant NPs, it is challenging to produce these expensive molecules and difficult to advance the discovery of novel plant NPs from the rapidly growing transcriptome data. Baker’s yeast Saccharomyces cerevisiae has been demonstrated to be a promising biotechnological production platform for plant natural products. Thus, S. cerevisiae has been considered to be a promising heterologous host to simultaneously reconstitute and elucidate plant natural product biosynthesis. Here, through a combination of metabolic engineering, strain evolution, and pathway reconstruction, a yeast-based system was developed to reconstitute and thereby elucidate the biosynthesis of phytosteroid and derivatives.

Development of a yeast-based system to elucidate phytosteroid biosynthesis

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.