Due to the increased performance of novel upstream processes, continuous downstream processes, which allow higher throughput, are gaining more importance in industry, since they increase yield and decrease process time manifolds. Hence, further efficient technologies are being developed recently.<br/>Among new emerging technologies in downstream processing, membrane chromatography holds excellent potential for the purification of proteins. Their high level of downstream productivity and versatility is achieved by combining the functionality of resin-based columns with the high flow rates of filter membranes. The major applications of membrane chromatography are the purification of large biomolecules like viruses, VLPs and protein complexes, and the removal of contaminants like DNA, HCPs etc. in flow-through mode.<br/>In this study, a prototype ion-exchange membrane was used for high resolution separation of protein variants in a bind and elute mode. As a midsize model protein, a monoclonal antibody with three charge variants was used at low and high protein loadings. To elucidate the elution behavior of the three monoclonal antibody charge variants, mechanistic modeling was applied using linear pH and salt gradient elution experiments. Utilizing the model parameters in <i>in-silico</i> simulations, chromatograms are predicted and the separation has been optimized. The performance and binding capacity of the membrane is compared to a commercially available resin-based column.

High resolution membrane chromatography: Modeling and simulation of mAb charge variant separation

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.