While the scientific community has spent decades working towards understanding biofilm formation and composition, the influence of signaling pathways and individual proteins on biofilm is still fertile ground for investigation. We utilize the exogenous overexpression of specific signaling pathways to determine their impact on biofilm formation and maturation. We characterize the impact of overexpressing fliA, a transcription factor, on biofilm by analyzing extracellular structures and biofilm composition using traditional biochemical techniques such as immunoblotting, as well as confocal and scanning electron microscopy. We also explore the influence of the downstream target phosphodiesterase, yhjH, on c-di-GMP signaling and biofilm formation at various temperatures. We aim to provide the field with a greater understanding of the relationship among specific signaling pathways and biofilm and a catalogue of targets that could be useful in the design of novel anti-biofilm agents.

Investigating the impact of fliA overexpression on the formation and composition of <i>E. coli</i> biofilms at different temperatures

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

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Investigating the impact of fliA overexpression on the formation and composition of E. coli biofilms at different temperatures