microRNAs are short, noncoding RNAs (~22-25 nucleotides) that negatively regulate translation by binding to the 3’ untranslated region of mRNA. Mature microRNAs are generated post-transcriptionally, starting with cleavage of the basal end of their initial transcript, primary microRNA (pri-microRNA). This is mediated by the enzyme Drosha to create a shortened stem-loop structure called preliminary microRNA (pre-microRNA). Recently, heterogenous nuclear ribonucleoprotein A1 (hnRNP A1), a RNA binding protein involved in many RNA related processes such as mRNA splicing, was found to aid in the processing of pri-microRNA-18a. microRNA-18a is part of polycistron <i>mir-17 – 92</i>, that encodes a cluster microRNA genes. The polycistron has been found to be overexpressed in numerous cancers giving it the name of oncomiR-1. Herein, we report the development of an assay to identify a small molecule that will be used to probe the interaction of pri-microRNA-18a and hnRNP A1 to better understand its role in selectively facilitating the production of microRNA-18a.<br/>

Probing the pri-microRNA-18a – hnRNP A1 interaction

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.