With more than 600 identified disinfection byproducts (DBPs), chemical analysis of all compounds in a water sample is not feasible. In addition, analysis of individual chemicals does not account for toxicological effects of complex mixtures. In recent years, bioanalytical tools such as in vitro assays have been used as alternatives to analytical tools to assess water quality for potable reuse. Unlike in vivo assays with whole organisms, in vitro assays are often faster, require fewer resources, and work well for screening purposes. Therefore, in vitro bioassays could prove valuable for screening disinfected water samples. This work presents results from a recent study focused on bioassays suitable for screening disinfection byproducts (DBPs). Multiple bioassays (e.g., Chinese hamster ovary cytoxicity, Cell-Titer Blue, Mitochondrial Membrane Potential, umuChromotest and Glutathione-Glo) were evaluated for rapid screening of four classes of DBPs: trihalomethanes (THMs), haloacetic acids (HAAs), nitrosamines (NAs), and haloacetonitriles (HANs). These compound classes were chosen because they are regulated (THMs, HAAs) or proposed for regulation (NAs, HANs) due to significant toxicity. Bioassay responses varied; all assays exhibited a positive response for HANs and most exhibited a positive response for HAAs and THMs, while nitrosamines did not elicit bioassay responses. Additional results include effective concentrations (EC50) for individual chemicals, and an assessment of mixtures to determine if bioassay responses showed additive or antagonistic effects.

Alternative to analytical chemistry for assessing disinfection byproducts in potable water reuse

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.