Toxoplasma gondii (T. gondii), is a common food and waterborne apicomplexan parasite that causes the disease toxoplasmosis. Presently, there are few anti-toxoplasmosis drugs used for treatment. However, these drugs have been identified to have adverse clinical side effects, ineffective, highly expensive, and are not available in certain countries. Hence, new generations of effective and safe drugs are urgently needed to treat T. gondii infections globally. In this study, we tested the effect of apigenin, apigenin-7-O-glucoside, and pyrimethamine alone and in combination against T. gondii (RH-YFP) growth using both cell-based and fluorescent-based assays. Interestingly, the individual compounds (apigenin, apigenin-7-O-glucoside, and pyrimethamine) showed anti-T. gondii activity with EC50ps of 0.41 µg/mL, 0.80 µg/mL, and 0.64 µg/mL, respectively. Another important finding was that pyrimethamine combination with apigenin and pyrimethamine with apigenin-7-O-glucoside were highly effective in inhibiting parasite growth with EC50ps of 0.046 and 0.40 µg/mL, respectively. These compounds' anti-parasitic EC50ps concentrations were not cytotoxic to the human foreskin fibroblast (HFF) cell lines used.

Flavonoids-pyrimethamine based combination against Toxoplasma gondii growth

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.