Highly porous chitosan microspheres (CSM) were prepared through emulsion-based thermally induced phaseseparation (TIPS) without using toxic crosslinkers and chemical porogenic agents other than ice. The CSM had an average diameter of ∼150 μm with interconnected pores varying from 20∼50 μm in size. Due to their excellent biocompatibility and unique porous structure, high-performance hepatocyte culture in three-dimensional (3D) space was achieved using the CSM as microcarriers, as cell growth also took place within the internal pores of the CSM, besides their external surface, and multidirectional cell–cell interactions were observed. Enhanced cellular activity and functions were obtained with the CSM microcarriers as compared with 2D cell culture. It is believed that these CSM microcarriers provide a promising platform for 3D cell culture in vitro.<br/>Moreover, responsive microcarriers based on chitosan microspheres were prepared for cell detachment. The surface of chitosan microspheres was first modified with β-cyclodextrin (β-CD), then further modified with gelatin through the host-guest interaction between β-CD and aromatic residues in gelatin. Cell detachment was achieved by adding an appropriate amount of amantadine (AD) in the culture. This trypsin-free cell detachment could avoid the possible cellular damage and may have bright prospects in cell culture and relevant areas.<br/>

Chitosan microspheres as microcarriers for 3D cell culture and detachment

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.