Many agents in environment can result in DNA damage directly or through metabolic processes. 4-aminobiphenyl, a well-known aromatic amine carcinogen present in smoke, can be activated by N-acetyltransferase to from C8-substituted dG-ABP (major), N2-substituted dG-ABP and N6-substituted dA-ABP adducts.  These ABP lesions are bulky lesions which have been demonstrated to provide conformational heterogeneities and to affect DNA replication and repair.   <br/>In this study, we firstly synthesized and identified the C8-dG ABP adduct containing oligonucleotides. Following the Competitive Replication and Adduct Bypass (CRAB) and Restriction Endonuclease and Post-labeling (REAP) Assays, we examined the replication block and mutagenicity of the lesion and correlated with the conformations of the lesion in ds-DNA.  The results show that ABP caused the change of mutational spectra is sequence dependent.<br/>

Sequence effects of 4-aminobiphenyl DNA lesion-induced mutational spectra

ACS Fall 2020 Virtual Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.