The process of the tandem liquid chromatography-mass spectrometry method for the detection of nitrofuran metabolites in shrimp is described. A main concern with shrimp is the threat that they have been treated with nitrofuran antibiotics that are known human carcinogens. Testing for the nitrofuran tissue-bound antibiotic metabolites was done to the part per billion sensitivity level using both liquid chromatography and mass spectrometry after extracting about 0.2mL of proteins from a 2-gram shrimp sample. The process has three phases of preparation before the sample is analyzed by the chromatograph and mass spectrometer. These are compositing, derivitization and extracting. A recently improved method can be completed by one analyst in one day.

Detection of nitrofuran metabolites in imported shrimp: An internship experience at the Food and Drug Administration

240th National Meeting (2010)

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