trans-Resveratrol (trans-3,5,4'-trihydroxystilbene) exhibited inhibitory activity against mushroom tyrosinase (EC1.14.18.1) through a kcat inhibition mechanism. Tyrosinase oxidized resveratrol and multiple oxidation products were observed with high performance liquid chromatography (HPLC) analysis. In the inhibitory assays, resveratrol did not inhibit the diphenolase activity of tyrosinase when L-3,4-dihydroxyphenylalanin (L-DOPA) was used as a substrate; however, L-tyrosine oxidation by tyrosinase was suppressed in presence of 100 �M of resveratrol. Oxidation of resveratrol and inhibition of L-tyrosine oxidation suggests the kcat inhibition of resveratrol against tyrosinase. After tyrosinase was pre-incubated with resveratrol, both monophenolase and diphenolase activities of tyrosinase were significantly reduced, which indicates kcat inhibition. Furthermore, investigation was extended to the cellular experiments by using B16-F10 murine melanoma cells. Cellular melanin production was significantly suppressed by resveratrol without any cytotoxicity up to 200 �M. of it. trans-pinosylvin was used as a comparison. These results suggest that possible usage of resveratrol as a tyrosinase inhibitor and a melanogenesis inhibitor.

Resveratrol as a <i>k</i><sub>cat</sub> type inhibitor of tyrosinase enzyme: Potentials as a melanogenesis inhibitor

240th National Meeting (2010)

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Resveratrol as a kcat type inhibitor of tyrosinase enzyme: Potentials as a melanogenesis inhibitor

Resveratrol as a k_cat type inhibitor of tyrosinase enzyme: Potentials as a melanogenesis inhibitor