The concentration of product intermediates is necessary or useful in a number of biopharmaceutical processing applications. However, traditional fed-batch tangential flow ultrafiltration (TFF) can be limited due to the size and minimum working volume of TFF systems. In these situations, single-pass tangential flow ultrafiltration (SPTFF) provides a viable solution by providing high concentration factors without the need of a recirculation vessel. This reduces overall system size and hold-up and enables concentration in-line with other operations. Our team examined the performance SPTFF in multiple bioprocessing applications and demonstrated robustness across several products. Integration and scale-up into existing manufacturing processes is explored and process economics are discussed. Results illustrate the benefits of SPTFF as a platform purification technology.

Integrating single-pass tangential flow filtration into biopharmaceutical purification processes

243rd National Meeting (2012)

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