The initial genome-scale reconstruction of the metabolic network of Escherichia coli K-12 MG1655 was assembled in 2000. It has been updated and periodically released since then based on new and curated genomic and biochemical knowledge. An update has now recently been built, named iJO1366, which accounts for 1366 genes, 2251 metabolic reactions, and 1136 unique metabolites. Like its predecessors, the iJO1366 reconstruction is expected to be widely deployed for studying the systems biology of E. coli and for metabolic engineering applications. The procedures and approaches used to update the reconstruction in the era of omics data sets will be presented. Further, the use of the reconstruction towards guided design of networks for metabolic engineering purposes through simulation and integration with various omics data types will be discussed.

Comprehensive reconstruction of E. coli metabolism and application to model-driven metabolic engineering and omics data analysis

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.