The objectives of this study were to evaluate the effect on markers of inflammation using lipopolysaccharide (LPS)-induced RAW 264.7 macrophages of protein hydrolysates of the common bean (Phaseolus vulgaris L.) varieties Negro 8025 and Pinto Durango. Cell viability was determined and the percentage of viable cells was calculated and concentrations that allowed >80% cell viability were used to determine markers of inflammation. Alcalase hydrolysates of common beans inhibited cyclooxygenase-2 expression, prostaglandin E2 production, inducible nitric oxide synthase expression and nitric oxide production, for Pinto Durango, with IC50 values of 34.9 � 0.3, 13.9 � 0.3, 5.0 � 0.1 and 3.7 � 0.2 �M, respectively while var. Negro needed 43.6 � 0.2, 61.3 � 0.3, 14.2 � 0.3 and 48.2 � 0.1 �M. In conclusion, hydrolysates from common beans can be effectively used as ingredients to reduce inflammatory associated diseases.

Bean concentrates and inflammation reduction

243rd National Meeting (2012)

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