Nanoparticles provide an ideal remedy to usually contradictory issues between minimizing diffusion impact and maximizing the enzyme activity for enzyme immobilization. In this study, a flame spray pyrolysis method was employed for the synthesis of nonporous, silica nanoparticles. Properties of the silica nanoparticles were characterized using TEM, XRD and FTIR analyses. A covalent coupling method was developed for immobilizing a Carbonic Anhydrase (CA) enzyme onto the nanoparticles. Optimal immobilization conditions were experimentally identified to achieve the best tradeoff between high loading and high activity retention of the CA enzyme. The immobilized CA enzymes were evaluated for their activities for promoting the rate of CO2 absorption into a potassium carbonate solution under different pH and temperature conditions and classic Danckwerts theory for absorption with reaction was employed to determine their enzymatic kinetics. Initial results also revealed that the immobilized CA enzymes exhibited significantly improved thermal stabilities compared to the free counterpart.

Immobilization of a carbonic anhydrase enzyme onto flame spray pyrolysis-based silica nanoparticles for promoting CO<sub>2</sub> absorption into a carbonate solution for post-combustion CO<sub>2</sub> capture

243rd National Meeting (2012)

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Immobilization of a carbonic anhydrase enzyme onto flame spray pyrolysis-based silica nanoparticles for promoting CO2 absorption into a carbonate solution for post-combustion CO2 capture

Immobilization of a carbonic anhydrase enzyme onto flame spray pyrolysis-based silica nanoparticles for promoting CO₂ absorption into a carbonate solution for post-combustion CO₂ capture