In this paper, a novel strategy is proposed to rapidly detect HBV in high-throughput by combining bioinformatics, chemiluminescene, concentration on magnetic particles and whole genome amplification. To reach the goal, functional magnetic particles with core-shell structure are prepared. The specific probes of HBV are immobilized on modified magnetic nanoparticles with core-shell structures. The target fragments are prepared by whole genome amplification from the HBV DNA from monomers with biotin. After hybridization, the target fragments are captured on magnetic nanoparticles carrying the specific probes and the nonspecific adsorbed fragments are removed under magnetic fields. For the later, the captured target fragments are connected to an alkaline phosphatase center modified with avidin. The chemiluminescent signals are produced and analyzed by introducing a chemiluminescent substrate(AMPPD) to the well containing the complex of magnetic nanoparticles and target fragments with alkaline phosphatase center modified with avidin.

Chemilumiscene detection of hepatitis B virus(HBV) based on magnetic nanoparticles and whole genome amplification

243rd National Meeting (2012)

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