Advances in mammalian cell culture processes have necessitated the development of harvest technologies that are capable of clarifying cell culture feed stocks with high cell densities or solid contents. This has required improved understanding and utilization of fundamental separation principles. The speed and efficiency of harvest processes are influenced by a number of factors including the nominal particle size and particle size distribution of the suspended solids. The size of the suspended solids can be modulated through pre-harvest additions, such as flocculants, or by processing conditions, such as mixing or pumping. Thus, in optimizing harvest processes, it is critical to understand the factors that change the suspended solids particle size.The use of commercially available particle sizing technologies, including the Focused Beam Reflectance Measurement technology, in the development of a flocculant harvest process will be discussed.  Applications of particle sizing data to predict optimal flocculant dosing and depth filtration capacity will also be discussed.

Applications of particle sizing techniques in mammalian cell culture harvest process development

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.