Corner cube retroreflectors (RR) are objects with three mutually perpendicular reflective surfaces that return light directly to its source and are therefore extremely bright and detectable using simple, low-cost optics. In this work, we report the lithographic fabrication of suspended five micron corner cube retroreflectors and their use as ultra-bright labels in a rapid pathogen detection assay. In preliminary experiments, the RR cubes were functionalized with analyte specific antibodies and combined with the analyte (E. coli bacteria) and magnetic sample preparation particles modified with analyte-specific antibodies. We optically tracked the magnetic �drag� of suspended cubes facilitated by analyte-driven binding of magnetic particles onto the cubes. We further explore assay integration with microfluidics for the development of a portable and automatable diagnostic assay platform.

Suspended micron sized corner cube retroreflectors as ultra-bright labels for pathogen diagnostics

243rd National Meeting (2012)

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