Owing to its ability to obtain molecularly specific contrast based on the vibrational spectra of molecules in the specific sample, Coherent Anti-Stokes Raman Scattering (CARS) microscopy has developed as a powerful label-free microscopy technique. It has been challenging, however, to use CARS in a quantitative manner, especially for microscopy employing single-frequency CARS.  One of the key advantages of multiplex CARS is the possibility to monitor and quantify small changes in the local chemical composition of a sample with submicron resolution. The non-resonant background is used to heterodyne the CARS signal, and a simple phase retrieval method provides the corresponding, quantitative, local Raman response. We report the use of quantitative multiplex CARS microscopy to important challenges in material- and bio-sciences.

Quantitative multiplex CARS microscopy

244th National Meeting (2012)

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