Liquid chromatography tandem mass spectrometry is typically the method of choice for quantitation of trace level analytes in biological samples.  However, this approach often is limited by the presence of chemical noise and/or coeluting substances.  We investigate the potential of high resolution mass spectrometry to address these limitations. The quantitation of 7-ethyl-guanine in hydrolyzed DNA is used to test the full scan, selected ion monitoring, and product scan modes with an Orbitrap mass spectrometer at a resolution of 50,000 and extracted mass tolerance of =5ppm.  In addition, the quantitation using �low resolution� MS3 ion trap and MS2 triple quadrupole methodologies is compared to the �high resolution� modes under identical liquid chromatography and ion source conditions.  Limits of detection and quantitation of 10 and 50 attomole on-column, respectively, were determined under high resolution product scan operation.

Quantitation of DNA adducts using high resolution mass spectrometry: A case study

244th National Meeting (2012)

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