Lactobacillus acidophilus, Lactobacillus reuteri, Bifidobacterium longum and Bifidobacterium breve were inoculated into alginate-based synbiotic matrices that included fructo-oligosaccharides, inulin and pectic-oligosaccharides stored under aerobic refrigerated conditions for 28 days.  When calcium (45 mM) was used to cross link the alginate matrix, 2-3 log CFU/mL of L. acidophilus and L. reuteri, and 0-3 log CFU/mL of B. breve and B. longum survived. Acetic (3-10 mM), butyric (0-3 mM), propionic (5-16 mM) and lactic acids (30-60 mM) were produced during the anaerobic growth of the recovered probiotics in BHI broth at 37� C, confirming their metabolic activity. When calcium cross-linking was not used in synbiotics, the matrix was more gel-like and at least 7 log CFU/mL of probiotic bacteria survived after 21 days of aerobic refrigerated storage. Significantly higher levels of B. breve, L. acidophilus and L. reuteri survived in fructo-oligosaccharide, inulin and pectic-oligosaccharide synbiotic matrices compared to alginate alone.

Survival of probiotic bacteria within a synbiotic matrix

244th National Meeting (2012)

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