Umbilical cord blood (UCB) is an attractive source of hematopoietic stem cells (HSCs) for HSC transplantation. We investigated the ex vivo expansion of HSCs cultured on biomaterials grafted with several nanosegments, i.e., polyamine, fibronectin, RGDS, and CS1 (EILDVPST), at several surface densities. No direct correlation was found between fold expansion of HSCs and physical parameters of the culture dishes. However, a small amount of grafted amino groups (0.8 residual �mol/cm2) on the dishes was effective for the ex vivo expansion of HSCs. A high amount of grafted amino groups hindered the ex vivo expansion of HSCs on the dishes. HSCs cultured on dishes with a high concentration of CS1 (2.40 residual �mol/cm2) showed greater expansion of HSCs and more pluripotent colony-forming units than those on fibronectin-grafted and polyamine-grafted dishes. The specific interaction between HSCs and CS1 helps to maintain the pluripotency of HSCs during the ex vivo expansion of HSCs  .

Effect of the surface density of nanosegments immobilized on culture dishes on ex vivo expansion of hematopoietic stem and progenitor cells from umbilical cord blood

244th National Meeting (2012)

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