Slip flow gives narrower peaks in RPLC because it flattens the parabolic flow profile. This makes it especially attractive for separations of large protein drugs, such as monoclonal antibodies and their aggregates. The term slip flow refers to the nonzero velocity of the mobile phase at the surface of the stationary phase, which is what gives rise to the narrower velocity distribution. Slip flow becomes significant for hydrophobic particles below 1 mm in diameter, where it comprises most of the flow rate. We describe the experimental characterization of slip flow, and we present separations of proteins using particles of 0.5 and 0.25 um in diameter. The results show extremely efficient protein separations and healthy flow rates.

Slip flow in reversed phase liquid chromatography

245th National Meeting (2013)

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