Patients with diabetes type -2 and end stage renal disease accumulate marked increases in the levels of advanced glyccosylation endproducts (AGEs). Enhanced non-enzymatic glycation of proteins has been implicated in the development of macro-and microvascular complications in diabetes type-2 with accelerated atherosclerosis. We recently evaluated (ACS 2012, ACS AGFD 2012) the glycation inhibitory effects of bioactive compounds present in Okra Seed Extracts (OSE) and aminoguanidine a well-known glycation inhibitor by a fluorescent method. Percent inhibition was calculated to be 88 % for OSE and 86% for aminoguanidine. Thus bioactives in OSE were comparable to aminoguanidine which prevents the formation of reactive dicarbonyls which get converted to AGEs. Furthermore, Okra vegetable has the highest binding capacity (16%) with bile acids (degradation products of cholesterol) and thus conversion of cholesterol to bile acids and its rate of elimination from the body takes place rapidly. During this process, cholesterol 7alpha-hydroxylase the rate limiting enzyme in bile acid biosynthesis is accelerated thus enhancing the elimination of LDL-cholesterol. This information will be helpful in studying diseases such as diabetes type-2, atherosclerosis, gallstone disease and lipid storage diseases as evidenced from our laboratory (Dayal, Salen et al. J Lipid Res. 1983, Steroids, 1990, Salen, Dayal et al. J. Lipid Res. 1885, J. Clin. Invest. 1985,1979, Shefer and Dayal J Clin. Invest. 1976, Analytical Biochem. 1982.

Okra seed phytochemicals: Potential regulators of enzyme cholesterol 7-alpha-hydroxylase in bile acid biosynthesis

245th National Meeting (2013)

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