Metabolic Engineering aims to purposeful design or redesign biological system for the production of commercially valuable chemicals such as biofuels, platform chemicals and biologically active compounds. To achieve the successful design or redesign of the biological systems, robustness of naturally occurring biological systems has to be relieved so that cells can be easily redesigned. In this study, we developed the general tools to modify the biological robustness at the various levels including translation and protein levels. For translation level control, a model to predict the translation efficiency based on the mRNA's secondary structure and consequently expression level can be precisely controllable for any genes of interest. Additionally, intracellular metabolite sensor named �riboselector� to regulate metabolic distribution will be presented. The potentials of the platform technology developed in this study for the application to the production of biofuels and commodity chemicals.

Synthetic RNA devices for pathway design and evolutionary metabolic engineering

245th National Meeting (2013)

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