The ganglioside GM1a is widely accepted to be the sole binding partner for cholera toxin subunit B (CTxB). This assumption informs interpretation of studies of cholera toxin function, endocytic mechanisms, and lipid raft visualization. Using a metabolically incorporated photocrosslinking sialic acid analog we developed , we obtained evidence that CTxB also binds a glycoprotein(s) expressed on the surface of an intestinal epithelial cell line. I will describe our efforts to identify this glycoprotein and determine its role in cholera toxin function.

Discovery of novel toxin binding partners using photocrosslinking sialic acid

245th National Meeting (2013)

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