Bone morphogenetic protein 2 (BMP-2) is a member of the TGF-� growth factor family that exhibits multiple functionalities in the process of bone remodeling.  As such, surface modification of intrinsically bioactive ceramic scaffolds with BMP-2 could serve to further enhance the osteogenic effects of the scaffolds at a bone defect site.  In this study, calcium aluminate and calcium phosphate scaffolds were modified with BMP-2 by direct surface adsorption or chemical immobilization utilizing an organic linker system.  The effect of surface modification was evaluated by human osteoblast growth at Days 1, 4, and 7 using a Live/Dead Cytotoxicity assay. Differentiation of adult mesenchymal stem cells was evaluated on the substrates using an alkaline phosphatase assay.  It was shown that the method of biomolecule attachment as well as scaffold composition influenced cell viability and differentiation and that chemically immobilized BMP-2 on CaP scaffolds promoted cell viability to the highest degree.

Chemical immobilization of bone morphogenetic protein 2 on ceramic scaffolding enhances substrate bioactivity

245th National Meeting (2013)

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