Injuries caused by oxygen deficit and reperfusion are deleterious to the cells and are involved in serious illnesses including heart attack, stroke, Alzheimers, and others. During hypoxia, the mitochondrial matrix is swelling and cytochrome c (Cyt c) is released to the cytosol, marking the beginning of cell apoptosis. In this work, we have investigated electrostatic interactions of mitochondria with thiol-capped gold nanoparticles (AuNP) evidenced by the bathochromic shift of the local surface plasmon (SP) and changes in resonance elastic light scattering (RELS) intensity. The interactions of hemoprotein (Cyt c) and coenzyme Q with nanoparticles have also been investigated. The effect of various drugs influencing the potassium ion-channel opening and inner mitochondrial membrane polarization have been analyzed to elucidate the mechanisms of reduced sensitivity of some cells to hypoxia and reperfusion conditions. The RELS spectroscopy and fluorescence spectroscopy have been employed in measurements.

Interactions of gold nanoparticles with mitochondria and Cyt c using resonance elastic light scattering and fluorescence techniques

245th National Meeting (2013)

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