Soy protein isolate (SPI), has the highest protein content and wide range of health benefits.In this study we aim to evaluate the anti-aggregative property of alcalase hydrolysates of SPI. Since the anti-aggregative properties against Alzheimer's �-amyloid peptide (A� 1-42) depends on the peptide composition of the hydrolysates and the enzyme used, the effect of process conditions on SPI hydrolyzed with alcalase was investigated systematically using response surface methodology. Hydrolysis conditions for optimal �-amyloid aggregation inhibition were defined using the response surface model of central composite design (CCD). The optimum conditions obtained by the model for hydrolysate (with maximum anti-beta amyloid aggregation activity) preparation was found to be pH=8, Time=1 hr, E/S=0.5%,Temperature= 50�C which was in perfect correlation with the experimental model. This is by far one of the first studies to show that soy protein hydrolysates possess bio-activity against beta amyloid peptide found in patients with Alzheimer's disease.

Optimization of preparation of soy protein hydrolysates using response surface methodology with Alzheimer's β-amyloid (Aβ 1-42) peptide aggregation inhibition property

245th National Meeting (2013)

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