Biopharmaceutical compounds such as recombinant viral protein antigens and saponin adjuvants are candidates for vaccination and treatment of virally induced cancers. These samples were analyzed by terahertz time domain spectroscopy on a standard polyethylene background.  Aliquot 100 �L solution of each was dispensed on the PE card, allowed to dry overnight at room temperature.  Dry sample weight was determined by a microbalance.  The terahertz spectrometer was calibrated with respect to a blank polyethylene card.  The biopharmaceutical samples spectra were then acquired one at a time (Fig. 1).  It was found that the compounds under study exhibit rich spectral features over the range of 0.1THz to ~34THz (Fig. 2).  Some of these peaks have been attempted to explain in terms of their molecular structure and chemical properties.  These results will be discussed with exemplary spectra. Interferogram of the buffer on PE card Close-up of Fourier transform magnitude spectra of 3 samples. The low-frequency PE peak is at ~71 cm-1 (2.14 THz).

Terahertz spectroscopic characterization of protein vaccine antigens and saponin-derived adjuvant macromolecules

246th National Meeting (2013)

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