The basics of surface-enhanced Raman spectroscopy (SERS) will be discussed in the context of enhanced sensing as a tool to monitor chromate reduction sites in remediating Shewanella oneidensis. Chromate reduction regions in single bacterium will be elucidated using chromate coated gold nanoparticles and intracellularly grown gold nanoislands. Next we extend this concept for the multiplex detection of chromate, sulfate, nitrate and reduced trivalent chromium in the same sample. In conclusion, we show that SERS imaging was able to validate two observations made by previous studies on chromate/sulfate and chromate/nitrate interactions in MR-1 biofilms.

Nanomaterials as intracellular detectors to monitor chromate reduction in remediating bacterium

246th National Meeting (2013)

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