The multiple mechanisms of interaction on one stationary phase give a wider range of applications compared to reverse phase or ion exchange separations. Non-porous submicron silica particles offer advantages such as increased speed and superior resolution when used as a stationary phase in chromatography. The modification for the mixed stationary phase was carried out by bonding an alkyl group to offer hydrophobic interaction and amine functional group which offers ion exchange interaction on the surface of non-porous submicron silica. Chromatographic performance was evaluated using ribonuclease A as a model protein. Two additional isoforms of this protein were observed using this stationary phase as compared to a commercial C4 column, which showed only one peak.

Mixed stationary phase using submicron silica particles for improved liquid chromatography of proteins

246th National Meeting (2013)

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