This presentation will display a panel of bioanalytical devices resulting developed through my work at the interface Chemistry-Physics-Biology in different research groups: (i) Microbiological paper-based platforms in Derda group, University of Alberta, Canada; (ii) Electrochemical paper-based devices in Whitesides group, Harvard University, USA; (iii) Optoelectrochemical sensor arrays (PhD projects) under Sojic supervision, University of Bordeaux, France.These latter high-density biosensing platforms were fabricated by nanostructuring one face of coherent optical fiber bundles using chemical etching, and functionalizing them with biological probes. One fluorescent DNA nanosensor array, where DNA probes were immobilized by electrodeposition of a polypyrrole, enabled the detection of the hybridization remotely through the imaging fiber.[1] We addressed different DNA sequences onto the same nanostructured array using electrochemical cantilevers.[2] The second opto-electrochemical biochip was a multiplexed sandwich immunoassay detected by electrochimiluminescent imaging resolved at the single bead level, a readout technique we optimized.[3] Both optical-fiber-based platforms demonstrate the potency of combining multiple physical and chemical techniques to obtain biochips with high-density and high-multiplexing potentials.At the other end of the spectrum of diagnostic devices, we can find portable, low-cost, simple and user-friendly platforms that will permit efficient and convenient analysis at the point-of-care. Electrochemical Microfluidic Paper-based Analytical Devices (E�PAD) used with commercial glucometer as a reader were developed for this purpose by wax-patterning and stencil-printing on paper. We demonstrated their application to quantify various analytes (e.g., glucose, lactate, cholesterol) in blood samples.[4]Examples of paper-based platforms for high-throughput testing of soluble compounds on cell cultures, and diagnostics using microorganisms (bacteria and bacteriophage) will also be presented.[1] Deiss, F. et al. Analyst, 2011, 136, 327-331[2] Descamps, E. et al. Lab Chip 2013, accepted, DOI: 10.1039/C3LC50335F[3] Deiss, F. et al. J. Am. Chem. Soc. 2009, 131, 6088-6089[4] Nie, Z. et al. Lab Chip 2010, 10, 3163-3169

Development of bioanalytical platforms using original substrates such as modified optical fiber bundles or patterned paper for applications in diagnostics

246th National Meeting (2013)

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