Abstract Health impact of nanoparitcles has become a public concern with the great advances of nanoparticles for various applications. Coating agents are an integral part of nanoparticles, but little attention has been paid during toxicity studies of nanoparticles. As a result, there are inconsistent toxicity results for certain nanomaterials. In this study, we explore the cycotoxicity of 11 commonly used coating agents using two cell lines: human epidermal (HaCaT) and lung fibroblast (CRL-1490) cells, and at concentrations of 3, 10, 30 and 100 �M, at two exposure time points: 2 h and 24 h, and with two different assays: MTT/MTS assay and Trypan Blue staining.  It was found that 6 of the 11 studied coating agents are cytotoxic. Especially those with long aliphatic chains surfactants, both the cationic aliphatic amine/ammonium compounds, cetyltrimethylammonium bromide, olyelamine, tetraoctylammonium bromide, and hexadecylamine, and the anionic sodium dodecylsulfate, are the most toxic. In addition, exposure time, test methods, and use of different cell lines also affect the cytotoxicity results.  Therefore, for nanoparticle toxicity tests, the complex factors for the test of a mixture, rather than for a pure compound, must be considered.  The use of different test methods, cell lines, and exposure times must also be considered when comparing cytotoxicity results.

Cytotoxicity of nanoparticle organic surface coating agents

247th National Meeting (2014)

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