Multidrug-resistant (MDR) Salmonella enterica serover Typhimurium definitive type 104 (DT104) presents one of the greatest challenges in public health care in today's society. Due to the resistance to drugs, current technology cannot effectively kill the presence of MDR Salmonella inside of the human body. As a result, the development of physiologically benign and highly sensitive methods for the detection and/or killing of the aforementioned bacteria is in high demand. Driven by the negative heath impacts of these bacteria, we developed a single walled carbon nanotube (SWCNT) hybrid multifunctional popcorn shaped iron core-gold plasmonic shell approach to target and magnetically separate MDR Salmonella DT104.  After functionalization of the SWCNT through a 1,3-dipolar cycloaddition with methyl nitro acetate and subsequent SWCNT attachment of the gold shell-iron core nanoparticles and MDR Salmonella targeted antibodies, bacterial detection was achieved by the use of label free surface enhanced Raman spectroscopy (SERS), and via selective photothermal methods, destruction of the targeted bacteria was also accomplished.  In order to confirm the successful synthesis of the nanoparticles and nanohybrids, a combination of FTIR, SERS, UV Spectroscopy, and TEM, were used.  Experimental data show that our SWCNT hybrid antibody-conjugated popcorn shaped magnetic core-plasmonic shell nanoparticles can be used for targeted separation & SERS imaging of MDR Salmonella. A targeted photothermal experiment, using 670-nm light at 1.5 W/cm2 for 15 minutes, resulted in selective irreparable damage to most of the MDR Salmonella.Acknowledgments:   The project described was supported by the National Science Foundation (HBCURISE: HRD-1137763), the National Institutes of Health through the NCRR-RCMI (Award Number: G12RR13459), and NIH/NIMHD (Award Number: G12MD007581) for the use of the Analytical CORE Facilities.

SWCNT hybrid popcorn shaped magnetic core-plasmonic shell multifunctional nanoparticle for targeted magnetic separation and enrichment:  Label-free SERS imaging and photothermal destruction of multi drug resistant Salmonella

247th National Meeting (2014)

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