In a previous study, we established a method involving activation of GFP expression by an artificial RNA-responsive riboregulator system capable of detecting structural perturbations of RNAs in vivo. Using this new approach, we have been able to detect structural differences between RNA variants and RNA conformers by collecting flow cytometry data. In this talk, we will discuss how we have further optimized this platform for enhanced sensitivity. We will also present new findings that uncover how environmental and intracellular factors affect in vivo RNA folding in the context of the well-studied model Tetrahymena group I intron.

New fluorescence-based approach to capturing <i>in vivo</i> structural reconfigurations

247th National Meeting (2014)

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New fluorescence-based approach to capturing in vivo structural reconfigurations