Delivering large quantities of high purity compounds in the shortest possible time is the goal of a purification chemist. Two of the most popular purification techniques are Automated Flash Column Chromatography (AFCC) and Preparative HPLC.  Traditionally, AFCC is characterized by the ability to load large amounts of material and short purification times, while Prep HPLC is valued for high resolution separations resulting in very pure products. As a result, AFCC is typically used as a complementary technique whereby the crude sample is enhanced to a higher level of purity before final purification using Prep HPLC.With new developments in AFCC instruments and cartridge media, the gap between 'high speed' flash purification and 'high efficiency' Preparative HPLC purification is rapidly shrinking. In many cases AFCC can deliver large quantities of product comparable in purity to Prep HPLC, with significantly less time and expense.In this study, we demonstrate the performance improvements of AFCC, based on recent developments in instrumentation and cartridge media, in terms of increased resolution, increased flexibility, and time, solvent and cost savings.

Recent developments in automated flash chromatography improve purity and productivity

247th National Meeting (2014)

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