Field-flow fractionation (FFF) is a flow-based one-phase separation methodology. It is capable of separating an enormous assortment of macromolecules and particles with a size range of 1nm to 100�m. FFF is one of the most versatile families of separation technologies known, and applicable within many scientific and technological disciplines that encompass studies of polymers, powders, emulsions, environmental colloids, nanoparticles and biomacromolecules.This talk will review the principles and theory of field-flow fractionation and introduce the various subtechniques which use flow, sedimentation, thermal and electrical forces to enact separation fields. Then, instrumentations of each subtechnique will be shown, and key applications will be reviewed for macromolecule and colloidal analysis in biological, biomedical, biopharmaceutical, material science and industrial contexts. Detector technology will also be explored including multi-angle light scattering (MALS) and inductively-coupled plasma (ICP) mass spectrometry.A schematic of the asymmetrical flow FFF experiment: 

Field-flow fractionation: A tutorial

247th National Meeting (2014)

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