The �holy grail� of particle sizing can be regarded as the accurate and precise determination of the particle size averages and distribution of a sample that is disperse in size, shape, structure, and chemistry, along with the determination of the averages, distributions, and mutual-interdependences of all these properties. While no single analytical technique will be able to provide all of these for every sample type, multi-detector hydrodynamic chromatography (HDC) has demonstrated the ability to measure a number of these properties for a variety of samples. Advantages of HDC, vis-�-vis size-exclusion chromatography or field-flow fractionation, include its being gentler than the former (thus minimizing the possibility of on-column, flow-induced analyte degradation) and more affordable and easily implementable than the latter.This talk will focus on the use of packed-column HDC for characterizing particle size, shape, molar mass, and structure, by combining the information obtained from detection methods such as refractometry, viscometry, and light scattering (both dynamic and static).Figure 1. Mechanism of separation in HDC (from Striegel & Brewer, Annu. Rev. Anal. Chem. 2012,  5,15).

Particle characterization by multi-detector HDC

247th National Meeting (2014)

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