Advances in cell culture expression levels are resulting in output titers of =10 g/L moving in to downstream purification processes. Due to the high cost of Protein A resins, the current industry is faced with a challenge to accommodate these higher titer processes without drastically increasing processing time or operating cost. To prevent a bottleneck at the Protein A step, a dual flow loading strategy was used in conjunction with a new generation high capacity Protein A resin (MabSelect SuRe LX) to increase overall capacity or throughput. The loading strategy was optimized using an empirical model to obtain dynamic binding capacities of > 65 g/L. This represents an approximately 60% increase in binding capacity compared to the control MabSelect Sure resin runs under conventional loading conditions. The increased capacity was achieved without significantly increasing total residence time and affecting product quality

Maximizing binding capacity on Protein A chromatography

247th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.